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cell fractions  (Qiagen)


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    Qiagen cell fractions
    Cell Fractions, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 40611 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fractionation+kit/RNeasy+Mini+Kit/pm42119558-247-8-21
    Average 99 stars, based on 40611 article reviews
    cell fractions - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Fractionation:

    Article Title: The Transient Receptor Potential (TRP) Channel TRPC3 TRP Domain and AMP-activated Protein Kinase Binding Site Are Required for TRPC3 Activation by Erythropoietin
    Article Snippet: .. C , subcellular fractionation of endogenous TRPC3 and TRPC6 in UT-7/Epo cells with the Qiagen fractionation kit. ..

    Article Title: Targeted Gene Inactivation of Calpain-1 Suppresses Cortical Degeneration Due to Traumatic Brain Injury and Neuronal Apoptosis Induced by Oxidative Stress
    Article Snippet: .. Mitochondrial Fractionation 6 h after the induction of apoptosis, cells were separated into mitochondria, nuclei, and cytosol fractions using a fractionation kit (Qiagen). .. Measurement of Caspase-3 Activity Six hours after the induction of apoptosis, the enzyme activity of caspase-3 was measured in the cell lysate using a caspase-3 colorimetric assay kit (R&D Systems; BF3100).

    Article Title: Targeted Gene Inactivation of Calpain-1 Suppresses Cortical Degeneration Due to Traumatic Brain Injury and Neuronal Apoptosis Induced by Oxidative Stress
    Article Snippet: .. Mitochondrial Fractionation—6hafter the induction of apoptosis, cells were separated intomitochondria, nuclei, and cytosol fractions using a fractionation kit (Qiagen). .. Measurement of Caspase-3 Activity—Six hours after the induction of apoptosis, the enzyme activity of caspase-3 was measured in the cell lysate using a caspase-3 colorimetric assay kit (R&D Systems; BF3100).



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    Image Search Results


    A. IHC staining for WEE1 in Human EAC tissue sections depicting strong cytosolic localization B. Quantification of the Cytoplasmic and Nuclear Signals from A using Cell Profiler C. Cell fractionation of FLO1 and OE33 EAC cell lines followed by western blot for WEE1, p84 (Nuclear marker) and ὰ-Tubulin (Cytoplasmic marker) D- Co-Immunofluorescence of WEE1 (green) and C-MYC (Red) along with DAPI nuclear stain (Blue) in EAC cell lines FLO1 and OE33, captured at 40X Magnification.E.MYC mRNA expression in WEE1 high Vs WEE1 low EAC tissue samples derived from TCGA and 4 different GEO datasets. F. Gene Set Enrichment Analysis (GSEA) of MYC target genes in WEE1 high Vs WEE1 low EAC samples G. WEE1 mRNA expression in non-cancerous normal esophagus (Normal) and Esophageal cancer (Tumor) tissue samples, analyzed by TNM plot.com . *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. H. MYC mRNA expression in non-cancerous normal esophagus (Normal) and Esophageal cancer (Tumor) tissue samples, analyzed by TNM plot.com . I. Co-Immunofluorescence of WEE1 (green) and C-MYC (Red) along with DAPI nuclear stain (blue) in normal esophagus and gastroesophageal adenocarcinoma tissue sections in TMA, captured at 20x magnification. J- Quantification of fluorescence intensity from I K – Correlation analysis between WEE1 and MYC signal intensity

    Journal: Cancer letters

    Article Title: WEE1 Stabilizes MYC to Promote Therapeutic Resistance in Esophageal Adenocarcinoma

    doi: 10.1016/j.canlet.2026.218418

    Figure Lengend Snippet: A. IHC staining for WEE1 in Human EAC tissue sections depicting strong cytosolic localization B. Quantification of the Cytoplasmic and Nuclear Signals from A using Cell Profiler C. Cell fractionation of FLO1 and OE33 EAC cell lines followed by western blot for WEE1, p84 (Nuclear marker) and ὰ-Tubulin (Cytoplasmic marker) D- Co-Immunofluorescence of WEE1 (green) and C-MYC (Red) along with DAPI nuclear stain (Blue) in EAC cell lines FLO1 and OE33, captured at 40X Magnification.E.MYC mRNA expression in WEE1 high Vs WEE1 low EAC tissue samples derived from TCGA and 4 different GEO datasets. F. Gene Set Enrichment Analysis (GSEA) of MYC target genes in WEE1 high Vs WEE1 low EAC samples G. WEE1 mRNA expression in non-cancerous normal esophagus (Normal) and Esophageal cancer (Tumor) tissue samples, analyzed by TNM plot.com . *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001. H. MYC mRNA expression in non-cancerous normal esophagus (Normal) and Esophageal cancer (Tumor) tissue samples, analyzed by TNM plot.com . I. Co-Immunofluorescence of WEE1 (green) and C-MYC (Red) along with DAPI nuclear stain (blue) in normal esophagus and gastroesophageal adenocarcinoma tissue sections in TMA, captured at 20x magnification. J- Quantification of fluorescence intensity from I K – Correlation analysis between WEE1 and MYC signal intensity

    Article Snippet: Cell fractionation in FLO1 and OE33 cell lines was carried out according to the protocol described in the Cell Fractionation Kit (CST).

    Techniques: Immunohistochemistry, Cell Fractionation, Western Blot, Marker, Immunofluorescence, Staining, Expressing, Derivative Assay, Fluorescence